Figure 4. The fluorescence signal is strong and highly dynamic in the apical dome of fluorescently labeled root hairs. Fluorescence images were subjected to deconvolution analysis using the near neighbor application (Metamorph, Molecular Dynamics). This processing revealed that the fluorescence signal was strongest in the apical dome in close proximity to the plasma membrane, that the signal was highly dynamic, and that the intensity of the signal oscillated as indicated by asterisks indicating when the signal is higher.