Figure 3. Itraconazole induces autophagy in vivo. (A) Nude mice with U87 subcutaneous tumor xenografts were treated with hydroxypropyl-cyclodextrin (vehicle, n = 9) or 75 mg/kg itraconazole (n = 9) twice daily by oral gavage for 3 wk. LC3 expression in tumor xenografts was examined by immunohistochemistry. Eight individual fields of each slide were randomly selected for evaluation of the intensity of LC3 staining. (B) Conversion of LC3-I to LC3-II in tumors from vehicle- or itraconazole-treated mice was examined by immunoblot. Representative image of LC3 immunoblots was shown. Itra, itraconazole.