Skip to main content
. 2004 Jun 1;18(11):1317–1330. doi: 10.1101/gad.1165204

Figure 2.

Figure 2.

Wild-type and p53-/- SF-MEFs have stable karyotypes. Embryo fibroblasts at the indicated PD were harvested for karyotype analysis. (A) Wild-type MEFs at PD 3. (B) Wild-type MEFs at PD 15. (C) Postcrisis wild-type MEFs at PD 30 (these cells had doubled ∼15 times from the first splitting of a near-confluent flask of spontaneously immortalized cells that escaped senescence). (D) Wild-type SF-MEFs at PD 60. (E) p53-/- MEFs at PD 3. (F) p53-/- MEFs at PD 30. (G) p53-/- SF-MEFs at PD 60. (H) p53-/- SF-MEFs at PD 60 were supplemented with 10% FBS for 48 h and then prepared for metaphase spreads as outlined in Materials and Methods. Approximately 50 metaphases from two representative cultures were counted in each case. The exception was the wild-type MEFs at PD 15, where a total of 54 metaphases were counted from two representative cultures. These cells were approaching senescence, which limited the number of available metaphases.