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Expression of oncogenic Ras elicits a DNA-damage-like response leading to the induction and phosphorylation of p53. Wild-type SF-MEFs were infected with vector control or H-RasV12-expressing retrovirus. The indicated cells were simultaneously treated with 5 mM NAC. Cells were harvested ∼24 h after infection. Total cellular lysates were immunoblotted for total p53 and Ser 18-phosphorylated p53. Uniform loading of cell lysates was confirmed by immunoblotting for tubulin.
