Figure 2. Growth and expression of defense-related genes (Br-PR1) in transgenic Brassica rapa plants under normal growth condition. (A) Each image shows 25-d-old T3 transgenic B. rapa that carried both RPS4 and RRS1 (R + R), RPS4 alone, RRS1 alone, and wild type control plants (WT). (B) Five leaf disks were cut from the leaves of WT and transgenic B. rapa plants, and total RNA was isolated for qRT-PCR analysis. Expression level of Br-CBP20 in B. rapa was used for normalization.²⁷Br-PR1 expression is shown relative values.²⁷ Bars indicate SE. The data indicate no significant differences compared with the wild type controls (Dunnett’s method, P < 0.05).²⁸ The experiment was repeated twice with similar results.