Figure 2. Effect of 2-PCPA on H3K4me2 occupancy in promoters of defense related genes. Five-week-old wild type plants were infiltrated with either 10mM MgCl₂ (M) or 10 mM of 2-PCPA. Three days later upper untreated leaves were harvested, and chromatin isolated from these leaves was precipitated with anti-H3K4me2 antibody. Abundance of individual target loci (relative to ACTIN 2) that were associated with H3K4me2 was determined by real-time PCR. Each bar represents the mean ± SD (n = 3). Different letters above the bars indicate values that are significantly different (P < 0.05) from each other as determined by one-way ANOVA (Holm-Sidak method).