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WT p53 is functional in the UCI-107 cell line. (a) The expression of p53 targets (p21 and puma) and p53 itself was measured 10 hours post 8 Gy radiation. (b) WT p53 was stably knocked down using a shRNA in UCI-107 cell lines. (c) The clonogenic potential of UCI-107 cells was examined by measuring colony formation after treatment with the chemotherapy Cisplatin.
