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. Author manuscript; available in PMC: 2014 Oct 20.
Published in final edited form as: J Cancer Ther. 2014 May 16;5(6):506–516. doi: 10.4236/jct.2014.56058

Figure 4.

Figure 4

Mutant p53 proteins lose the interaction with the microprocessing Drosha complex. (a) Co-IP experiments demonstrated a loss of interaction with the Drosha complex in cell models expressing various mutated forms of p53. SKOV3 cell lines (p53 null) stably expressed p53 mutant proteins (6 oncomorphic mutations (R175H, R248Q, R248Q.P72R, R273C, R273L, and R273S) and 1 unclassified mutation (I195T). IP of DDX5 or p53 detected binding of p53 in the WT UCI-107 cell lines, but not in any other cell lines with mutant p53 proteins. (b) WT p53-responsive miRNA expression in SKOV3 cells expressing R248Q.P72R p53 mutant at a time course after radiation treatment.