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letter
. 2003 Feb 1;13(2):182–194. doi: 10.1101/gr.681703

Table 1.

Summary of Sequenced γ1230 Library Clones

Region Clones Sequences
no. avg. size (kb) total (kb)a contig size (bp)b type sequence (bp)c
Beagle (Motu 1) 16 2.8 52.5 8043 L AATAT 726
Beagle 7063
R AATAT 254
F (Motu 2) 17 3.3 71.4 6431 L AATAT 942
F 4713
R AATAT 776
412 (Motu 3) 32 2.7 82.4 9149 L AATAT 970
412 7572
R AATAT 607
Bel (Motu 4) 13 2.6 46.5 6888 L AATAT 582
Bel 6129
R AATAT 177
You (Motu 5) 5 1.7 24.7 7568 L AATAT 679
5333
R AATAT d950
R TTCTC d606
Maupiti 101 2.9 210.2 39,125 L TTCTC 201
Maupiti 38,924
Pure AATAT 2 1.5 3.7 e1058 AATAT e1058
Pure TTCTC 4 0.4 4.2 f1627 TTCTC f1627
CEN Subtotals 190 2.8 495.6 79,889 Complex 69,734
Satellite 10,155
Breakpointg 20 2.0 27 2655 Euchromatin 2291
R AATAT 364
Subtelomeric and Euchromatic 35 2.4 47.3 hNA hNA hNA
Unassigned 214 3.0 378.0 hNA hNA hNA
γ1230 Totals 459 2.6 947.9 82,544 Complex 72,025
Satellite 10,519
a

Total amount of sequence generated from all clones for each region.

b

Size of contig assembled for each region. L and R indicate position of the satellite to the left and right of the complex DNA (predominantly transposons), respectively (Fig. 2); see Figure 2 for transposon orientations.

c

Amount of satellite DNA sequence in the contig corresponding to the indicated junction.

d

At the 3′ end of the You contig, the transposon is juxtaposed to a 950-bp block of AATAT satellite, which in turn is juxtaposed with a block of TTCTC satellite.

e

Total for partial sequences from two clones, which both contained contigs of 529 bp.

f

Total for complete sequences from four clones, which contained contigs of 347, 439, 500, and 500 bp.

g

The breakpoint between the centromere and euchromatin produced during the generation of γ1230, see Figure 1. The AATAT satellite present in this clone marks the 5′ end of the centromere, but is not included in the CEN TOTALS for simplicity.

h

Assemblies of various sizes were produced, but are not included here because they are not relevant to centromere structure.