Table 1.
Region | Clones | Sequences | ||||
no. | avg. size (kb) | total (kb)a | contig size (bp)b | type | sequence (bp)c | |
Beagle (Motu 1) | 16 | 2.8 | 52.5 | 8043 | L AATAT | 726 |
Beagle | 7063 | |||||
R AATAT | 254 | |||||
F (Motu 2) | 17 | 3.3 | 71.4 | 6431 | L AATAT | 942 |
F | 4713 | |||||
R AATAT | 776 | |||||
412 (Motu 3) | 32 | 2.7 | 82.4 | 9149 | L AATAT | 970 |
412 | 7572 | |||||
R AATAT | 607 | |||||
Bel (Motu 4) | 13 | 2.6 | 46.5 | 6888 | L AATAT | 582 |
Bel | 6129 | |||||
R AATAT | 177 | |||||
You (Motu 5) | 5 | 1.7 | 24.7 | 7568 | L AATAT | 679 |
5333 | ||||||
R AATAT | d950 | |||||
R TTCTC | d606 | |||||
Maupiti | 101 | 2.9 | 210.2 | 39,125 | L TTCTC | 201 |
Maupiti | 38,924 | |||||
Pure AATAT | 2 | 1.5 | 3.7 | e1058 | AATAT | e1058 |
Pure TTCTC | 4 | 0.4 | 4.2 | f1627 | TTCTC | f1627 |
CEN Subtotals | 190 | 2.8 | 495.6 | 79,889 | Complex | 69,734 |
Satellite | 10,155 | |||||
Breakpointg | 20 | 2.0 | 27 | 2655 | Euchromatin | 2291 |
R AATAT | 364 | |||||
Subtelomeric and Euchromatic | 35 | 2.4 | 47.3 | hNA | hNA | hNA |
Unassigned | 214 | 3.0 | 378.0 | hNA | hNA | hNA |
γ1230 Totals | 459 | 2.6 | 947.9 | 82,544 | Complex | 72,025 |
Satellite | 10,519 |
Total amount of sequence generated from all clones for each region.
Size of contig assembled for each region. L and R indicate position of the satellite to the left and right of the complex DNA (predominantly transposons), respectively (Fig. 2); see Figure 2 for transposon orientations.
Amount of satellite DNA sequence in the contig corresponding to the indicated junction.
At the 3′ end of the You contig, the transposon is juxtaposed to a 950-bp block of AATAT satellite, which in turn is juxtaposed with a block of TTCTC satellite.
Total for partial sequences from two clones, which both contained contigs of 529 bp.
Total for complete sequences from four clones, which contained contigs of 347, 439, 500, and 500 bp.
The breakpoint between the centromere and euchromatin produced during the generation of γ1230, see Figure 1. The AATAT satellite present in this clone marks the 5′ end of the centromere, but is not included in the CEN TOTALS for simplicity.
Assemblies of various sizes were produced, but are not included here because they are not relevant to centromere structure.