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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: J Biomed Mater Res A. 2014 May 3;103(2):534–544. doi: 10.1002/jbm.a.35203

Fig. 1. Morphological and histological characterization of chondrocytes in AQ and HFIP silk scaffolds.

Fig. 1

(A) Bright field images of AQ and HFIP silk scaffolds. Top views (top panels) and side views (bottom panels) of the scaffolds. Scale bars: 2mm. (B) Scanning electron microscopic (SEM) images of AQ and HFIP silk scaffolds. Top panels: low magnification, scale bar: 200µm. Bottom panel, high magnification, scale bar: 40µm. (C) Low magnification SEM images of bovine articular chondrocytes inside the scaffolds after 16 days of culture, scale bar: 200µm. (D) High magnification SEM images of chondrocytes inside the scaffolds after 16 days of culture. Scale bar: 50µm. (E) H&E staining of chondrocytes in AQ and HFIP silk scaffolds. Scale bar: 25µm. (F) Toluidine Blue staining in chondrocytes in AQ and HFIP scaffolds. Scale bar: 25µm. For C–F: Ctrl (no cytokine added), IL-1β (10ng/ml), and TNFα (10ng/ml).