Figure 3. The mean square error of the RVE conformation deviation from its molecular conformation²⁶ across the membrane in the closed and open states of MscL; Mean Square Error is defined as $(MSE)=\frac{1}{n}{{\displaystyle {\sum }_{i=1}^n(R}}_i-R_i{(}^2$ Where ^R_i is the orientation of an alternative point across the pore and R_i is the orientation of the corresponding point on the relevant MD graph of the pore.²⁶n is the total number of points (nodes) which are distributed equally through the bilayer thickness (z coordinate) which was sufficient to be taken as 15. The unbroken line shows the sum of mean squares in the two (closed and open) states. E = 40 MPa would be the most appropriate value for the RVE Young’s modulus with respect to both closed and open conformations.