Figure 2. Channels from cells treated overnight with carbamazepine show diminished response to MgADP, an effect that is reversed after > 2-h washout of the drug. (A) Representative inside-out patch-clamp recordings testing MgADP responses of WT and F27S channels expressed in COSm6 cells treated overnight with 10 µM carbamazepine with (right) or without (left) 2 h of washout prior to recording. (B) Quantification of MgADP response. Currents in 0.1mM ATP or 0.1mM ATP plus 0.25mM ADP were expressed as percent of those observed in K-INT. Asterisks indicate statistical significance in MgADP response between no washout and > 2-h of washout of carbamazepine prior to recording (P < 0.05, n = 3) by 2-tailed, unpaired Student t test.