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. 2014 Jan 23;8(3):222–229. doi: 10.4161/chan.27662

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Copyright © 2014 Landes Bioscience

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graphic file with name chan-8-222-g4.jpg — Figure 4. Effect of Klotho coexpression on KCNQ1/KCNE1 protein abundance in the cell membrane of Xenopus oocytes. (A) Confocal microscopy of the KCNQ1/KCNE1 protein abundance in Xenopus oocytes injected with water (left), injected with cRNA encoding KCNQ1/KCNE1 alone (middle) or expressing KCNQ1/KCNE1 together with Klotho (right). The images are representative for 3 independent experiments. (B) Arithmetic means ± SEM (n = 55–76) of the chemiluminescence of KCNQ1-Flag/KCNE1 protein abundance in Xenopus oocytes injected with water (dotted bar), injected with cRNA encoding KCNQ1-Flag/KCNE1 alone (white bar), or expressing KCNQ1-Flag/KCNE1 with Klotho (black bar). * (P < 0.05) indicates statistically significant difference from the protein abundance in Xenopus oocytes expressing KCNQ1-Flag/KCNE1 alone.