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. 2014 Jan 23;8(3):222–229. doi: 10.4161/chan.27662

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graphic file with name chan-8-222-g5.jpg — Figure 5. Effect of treatment with recombinant Klotho protein on KCNQ1/KCNE1 protein abundance in the cell membrane of Xenopus oocytes. Arithmetic means ± SEM (n = 70–72) of the chemiluminescence of KCNQ1-Flag/KCNE1 protein abundance in Xenopus oocytes injected without (dotted bar) or with cRNA encoding KCNQ1-Flag/KCNE1 without (white bar) or with (black bar) a 24 h pretreatment with recombinant Klotho protein (30 ng/ml). *** (P < 0.001) indicates statistically significant difference from the protein abundance in Xenopus oocytes expressing KCNQ1-Flag/KCNE1 alone.