Figure 5. Acidotoxic cell swelling (NVI) and its sensitivity to hypothermia and anion channel blockers in cortical neurons. (A) Time courses of cell size (CSA) changes before (pH 7.4) and after application (started at arrows) of acidic (pH 5.25) solution at 25 °C (top) or 37 °C (bottom) in the absence (open symbols) or presence (filled symbols) of 10 μM DIDS or 50 μM phloretin. Insets: Representative microscopic images of a neuron before (0 min) and 2.5 min after the beginning of exposure to acidic solution at 25 °C (top photos) and 37 °C (bottom photos). (B) Summarized data showing the peak cell swelling (CSA recorded at 2.5 min) relative to the control cell size (CSA recorded at 0 min) at 25 °C (top) or 37 °C (bottom) in the absence (open column) or presence of 10 μM DIDS or 50 μM phloretin. Asterisks indicate significant difference (P < 0.01) between the data obtained at pH 5.25 without anion channel blockers and those obtained at pH 7.4 without anion channel blockers or at pH 5.25 with anion channel blockers. Daggers indicate significant difference (P < 0.01) between the data obtained at 25 and 37 °C.