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. 2014 Oct 20;9(10):e110464. doi: 10.1371/journal.pone.0110464

Figure 4. Immunoblotting and miRNA quantification of the in vivo data.

Figure 4

Data from immunoblotting (A–B) and miRNA quantification (C) associated to the Rac1-Cofilin pathway in hippocampus [H] and cortex [C] from NMRI mice exposed on postnatal day 10 with doses of 0 Gy and 1.0 Gy. The measurement was performed 24 hours post-irradiation. The columns represent the fold-changes with standard errors of the mean (SEM); immunoblotting: n = 4 for Rac1 detection; n = 3 for p-cofilin and cofilin detection; n = 3 for miRNA quantification. The visualisation of protein bands shows the representative change from the biological replicates. *p<0.05; **p<0.01; ***p<0.001 (unpaired Student's t-test). Normalisation was performed against endogenous GAPDH and endogenous snoRNA135 for immunoblotting and miRNA quantification, respectively.