Figure 2. Effect of UA on glucose uptake in 3T3-L1 adipocyte.

Mature 3T3-L1 adipocytes were incubated with 2.5 µM, 5 µM, or 10 µM of UA for 2 h and glucose uptake was measured in the presence or absence of 1 µg/mL insulin. The fluorescence intensity of NBD-glucosewas measured at 466/550 nm on a Varioskan Flash spectral scanning multimode plate reader. Data are expressed as means ± SD (n = 3). * P<0.05 and **P<0.01 vs. the control of 0 µM UA; ^#P<0.05 and ^##P<0.01 vs. 1 µg/mL of insulin.