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. 2014 Oct 20;9(10):e110711. doi: 10.1371/journal.pone.0110711

Figure 3. Effect of the PI3K inhibitor wortmannin on glucose uptake in 3T3-L1 adipocyte.

Figure 3

Mature 3T3-L1 adipocytes were incubated with 10 µM of UA for 2 h, or pretreated with 1 µM of wortmannin for 30 min before incubation with 10 µM of UA and 1 µM of wortmannin for 2 h. Glucose uptake was measured in the presence or absence of 1 µg/mL insulin. The fluorescence intensity of NBD-glucose was measured at 466/550 nm on a Varioskan Flash spectral scanning multimode plate reader. Data are expressed as means ± SD (n = 3). *P<0.05 and **P<0.01 vs. the control of 0 µM UA; #P<0.05, ##P<0.01 and ###P<0.001 vs. the insulin control of 1 µg/mL. Wor indicates 1 µM of wortmannin.