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. 2014 Oct 20;9(10):e110764. doi: 10.1371/journal.pone.0110764

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© 2014 Chang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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After a 14-day incubation in osteogenic medium, the expression of osteogenic differentiation markers by T1-P3, T3-P3 and T5-P3 cells was assayed using real-time RT-PCR (A–C). The y-axes (A–C) represent the expression rate of the target genes relative to that of 18S. At the same time, cells were lysed for western blotting. The expression of the osteogenic differentiation markers was detected in the three batches of cells (E). Bars represent the mean ± SD, n = 3. *p>0.05.