Table 1. Primers Used for RT-PCR and Real-Time RT-PCR.
| Part 1. Primers Used for RT-PCR | ||||
| Gene | Forward primer (5′-3′) | Reverse primer (5′-3′) | bp | Acc. No |
| ALP | ACGTGGCTAAGAATGTCATC | CTGGTAGGCGATGTCCTTA | 475 | NM_000478 |
| RUNX2 | CCACCCGGCCGAACTGGTCC | CCTCGTCCGCTCCGGCCCACA | 238 | NM_004348 |
| ON | ATGAGGGCCTGGATCTTCTT | GCTTCTGCTTCTGAGTCAGA | 580 | NM_003118 |
| COL II | TTTCCCAGGTCAAGATGGTC | TCACCTGGTTTTCCACCTTC | 498 | NM_033150 |
| COL X | GCCCAAGAGGTGCCCTGGAATAC | CCTGAGAAAGAGGAGTGGACATAC | 703 | NM_000493 |
| ACAN | TGAGGAGGGCTGGAACAAGTACC | GGAGGTGGTAATTGCAGGGAACA | 395 | NM_013227 |
| LPL | GTCCGTGGCTACCTGTCAT | AGCCCTTTCTCAAAGGCTTC | 717 | NM_000237 |
| Adipsin | GGTCACCCAAGCAACAAAGT | CCTCCTGCGTTCAAGTCATC | 272 | NM_001982 |
| PPAR-γ | GGAAAGACAACAAACAAATCAC | TGCATTGAACTTCACAGCAAAC | 414 | NM_005037 |
| β-actin | CTTAGTTGCGTTACACCCTTTCTTG | CTGCTGTCACCTTCACCGTTCC | 159 | NM_001101 |
| Part 2. Primers Used for Real-Time RT-PCR | ||||
| ALP | CCCACAATGTGGACTACCT | GAAGCCTTTGGGGTTCTTC | 143 | NM_000478 |
| RUNX2 | CGGAGTGGACGAGGCAAGAG | TGAGGAATGCGCCCTAAATC | 194 | NM_004348 |
| OC | CACTCCTCGCCCTATTGGC | CCCTCCTGCTTGGACACAAAG | 112 | NM_199173 |
| 18S | GTAACCCGTTGAACCCCATT | CCATCCAATCGGTAGTAGCG | 151 | NR_003286 |
ALP, alkaline phosphatase; RUNX2, runt-related transcription factor 2; ON, osteonectin; COL II, type II collagen; COL X, type X collagen; ACAN, aggrecan; LPL, lipoprotein lipase; PPAR-γ, peroxisome proliferator-activated receptor γ; OC, osteocalcin.