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. 2014 Oct 20;9(10):e110847. doi: 10.1371/journal.pone.0110847

Figure 2. Kinetic screening of 12G5 mAb binding to CXCR4-ACMs immobilized onto biosensor chips.

Figure 2

A: Ab was injected at increasing concentrations (6.25–400 nM) over 100 s, followed by a buffer wash (without regeneration) between injections (immobilization level: ca. 5000 RU; biotin/streptavidin immobilization). B. Saturation binding of 125-I SDF1α to CXCR4-ACMs. A dissociation constant of 8.4 nM was determined. C. The same series of measurements as shown in Fig. 2 A, conducted using immobilized VLPS (immobilization level: 5000 RU).