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. 2004 May 17;101(22):8289–8294. doi: 10.1073/pnas.0402625101

Fig. 2.

Fig. 2.

Effects of D408N trap gp43 on the size of the Okazaki fragments. Lagging strand synthesis was monitored at increasing trap gp43 concentrations (0 and 1.5 μM on the minicircle and 0, 64, and 128 nM on the TRFII). The Okazaki fragments synthesized on the minicircle and the TRFII substrate are shown in A and B, respectively. In B, the DNA products at 20, 40, 60, 90, 120, and 150 sec are shown in the presence of increasing trap gp43 concentrations. The arrows in B indicate the average Okazaki fragment size at each trap concentration. The size of the Okazaki fragments are shown for minicircle (C) at 0 (solid trace) and 1.5 μM (dashed trace) of trap gp43, and for TRFII (D)at 0 (solid trace), 64 (dashed trace), and 128 nM (dotted trace) trap gp43, respectively. The peaks in D with relative Rf (Rf) values of 0.03 and 0.26 are due to leading strand synthesis and strand displacement synthesis, respectively. Relative Rf values are defined as the ratio between the migrated distances and the length of the gel.