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DACH1 in association with c-Jun to repress cyclin D1 transcription. Western blot analysis of ACHN (A) and CAKI (B) cells expressing DACH1 wt, DS or vector control. C. RT-PCR detected cylin D1 and DACH1 mRNA abundance. D. Schematic structure of DACH1 and cylin D1 promoter. E. Luciferase activity of cyclin D1 promoter in renal cancer cell line CAKI (E) and ACHN (F). G. Immunoprecipitation and western blot of CAKI cells. H. ChIP of cyclin D1 promoter AP-1 site in CAKI cells.
