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. 2014 Oct 9;15(1):883. doi: 10.1186/1471-2164-15-883

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© Francis et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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In situ hybridization reveals species-specific patterns of localization in neuronal dendrites. Fluorescent Microscopy evaluation of biotin-conjugated oligoprobes on paraformaldehyde fixed 14-day cultured rat and mouse cortical neurons hybridized with nine biotin-conjugated oligoprobes detected with streptadivin-Alexa Fluor 568 (Invitrogen). For each probe images set, the small bottom left corner panels represent MAP2 immuno-staining. Scale bar = 20 μm. (A), Probes against SFRS16, ARHGDIA and HNRPK transcripts show higher dendritic localization in mouse neurons than in rat neurons (Red box). (B), Probes against ZFP410, COMMD3 and RSP6 transcripts show higher dendritic localization in rat neurons than in mouse neurons (Blue box). (C), Probes against UBA52, OLFM1 and H2AFZ transcripts show high dendritic localization in both rat and mouse neurons (Black box).