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. 2004 May 24;101(22):8313–8318. doi: 10.1073/pnas.0306709101

Fig. 1.

Fig. 1.

Generation of Noc2 knockout mice. (a) Schematic representation of the mouse Noc2 gene, targeting vector, and targeted allele. Exons are indicated by arrows. Neo and TK indicate a neomycin-resistant gene and a herpes simplex virus thymidine kinase gene, respectively. Restriction sites are indicated. The probe used for Southern blot analysis is shown. (b) Southern blot analysis of F2 offspring. Genomic DNA was digested with SphI and SspI and was hybridized with the probe. Lanes: +/+, wild-type; +/-, heterozygote; -/-, homozygote. (c) Northern blot analysis. Total RNA (15 μg) from the pituitary and adrenal glands of Noc2+/+ mice and Noc2-/- mice was used. (d) RT-PCR analysis of pancreatic islets of Noc2+/+ mice and Noc2-/- mice. Lanes: +/+, Noc2+/+; -/-, Noc2-/-. Noc2 transcript was not detected in Noc2-/- mice. (e) Western blot analysis. Homogenates of mouse pituitary gland, adrenal gland, and pancreas (20 μg) were subjected to SDS/PAGE and were immunoblotted with anti-Noc2 antibody [raised by immunizing rabbits with 17-mer peptide (QGGTPAQPEPRVPGKRH) corresponding to amino acid residues 279–295 of mouse Noc2]. Lanes: +/+, Noc2+/+; -/-, Noc2-/-.