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. 2014 Oct 10;13:133. doi: 10.1186/s12934-014-0133-0

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© Giardina et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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NAI-107 production in P0.1 (lighter gray bars), P0.5 (light gray bars), P5 (gray bars), P15 (dark gray bars) and P30 (black bars) media. The NAI-107 presence in the extracts was confirmed by LC-MS. The values were determined with a NAI-107 calibration curve (Additional file 1: Figure S3), as described in MM. Standard deviation was calculated from three independent bioassays. Asterisks indicate the phosphate concentration that allowed the maximum production.