Table 1.
Composition of media used for Microbispora fermentations
| Medium | Composition (g/L) | pH | References |
|---|---|---|---|
| GE82AB | Maltodextrin (20), Soy flour (15), yeast extract (5), CaCO3 (1), Agar (1). | 7.3 | Monciardini, personal communication |
| MG | Maltose (20), Glutamate (11.23), MOPS (21), MgSO4*7H2O (0.2), FeSO4*7H2O (0.09), CaCl2*2 H2O (0.001), NaCl (0.001), trace elements (as described for R2YE medium), 15 mM PO4 buffer. | 6.5 | Puglia et al. [24] |
| GG (20 or 50) | Glucose (20 or 50), Glutamate (11.23), MOPS (21), MgSO4*7H2O (0.2), FeSO4*7H2O (0.09), CaCl2*2 H2O (0.001), NaCl (0.001), trace elements (as described for R2YE medium), 15 mM PO4 buffer. | 6.5 | This study |
| NG (20 or 50) | Glucose (20 or 50), NH4NO3 (4), MOPS (21), MgSO4*7 H2O (0.2), FeSO4*7 H2O (0.09), CaCl2*2 H2O (0.001), NaCl (0.001), trace elements (as described for R2YE medium), 15 mM PO4 buffer. | 6.5 | This study |
| P0, P0.1, P0.5, P5, P15 and P30 | As NG20 with 0, 0.1, 0.5, 5, 15 and 30 mM of PO4 buffer, respectively. | 6.5 | This study |