Number of reads before trimming |
|
57,059,700 |
Number of reads after trimming |
|
52,770,704 |
Sequences discarded during trimming |
|
7.52% |
Average length before trimming |
|
94.5 bp |
Average lenth after trimming |
|
97.3 bp |
Number of reads per sample
|
|
|
T1 non-toxic strain-fed (AL1T) |
|
6,102,912 |
T1 toxic strain-fed (AL9T) |
|
14,571,224 |
T2 non-toxic strain-fed (AL1T) |
|
16,419,080 |
T2 toxic strain-fed (AL9T) |
|
15,678,278 |
Additional sequences used for the assembly
|
|
|
Illumina (digestive gland) |
|
49,871,662 |
454 (various tissues) |
|
115,557 |
Sanger (various tissues, Mytibase collection) |
|
18,788 |
Trinity assembly statistics
|
all contigs
|
longest transcript per gene
|
Assembly size |
16,350,006 bp |
11,571,682 bp |
Total number of contigs |
21,193 |
12,079 |
Mapping rate |
59.04% |
52.00% |
Non-specific matches |
4.29% |
0.00% |
N50 |
1,010 |
1,216 |
Mean contig length |
771 bp |
958 bp |
Longest contig |
14,931 bp |
14,931 bp |
Annotation statistics (longest transcript per gene model)
|
|
|
Contigs with BLAST hit vs UniProtKB/Swiss-Prot |
|
5,818 (48.1%) |
Contigs with BLAST hit vs C. gigas predicted proteins |
|
7,227 (59.8%) |
Contigs with BLAST hit vs P. fucata predicted proteins |
|
6,943 (57.5%) |
Contigs with BLAST hit vs L. gigantea predicted proteins |
|
6,699 (55.5%) |
Contigs with InterPro domains |
|
5,432 (45.0%) |
Contigs with PFAM domains |
|
5,696 (47.2%) |
Contigs with eggNOG terms |
|
4,524 (37.5%) |
Contigs with GO Cellular Component terms |
|
4,920 (40,7%) |
Contigs with GO Biological Process terms |
|
4,207 (34.8%) |
Contigs with GO Molecular Fuction terms |
|
4,236 (35.1%) |