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Interactions between M. tuberculosis NusA and the rrn leader region. (A) EMSA with the first half of the rrn leader region (+1 to +104) as probe. Lanes 1–4, 1 nM sense RNA; lanes 5–8, 1 nM antisense RNA. NusA concentrations are 5, 10, and 20 nM in 200 mM KCl. (B) EMSA with 1 nM nut RNA (+1 to +63). Lane 1, sense RNA; lanes 2–4, as described for lane 1 with 0.5, 1, 5, or 10 nM NusA; lane 5, antisense RNA; lanes 6–8, as described for lane 5 with 10, 25, 50 nM NusA, all in 1 M KCl.
