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Northern blot of untranslated lacZ expressed in M. smegmatis. The blot was probed with a probe complementary to nucleotides 1–125 of lacZ. Lane 1, KAM103 (Prrn-leaderwt-lacZ); lane 2, KAM107 (Prrn-nutwt-lacZ); lane 3, KAM109 (Prrn-lacZ); lane 4, KAM109, stained with ethidium bromide. Indicated transcript sizes are approximate and calculated from the positions of 16S and 23S RNA.
