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EMSA with E. coli NusA416 and the E. coli (E.c) rrnG leader nut RNA (nucleotides +1 to +64; lanes 1–5) and M. tuberculosis (M. tb) nut RNA (+1 to +63; lanes 6–10). Lane 1, E. coli rrnG; lanes 2–5, rrnG nut RNA with 5, 10, 25, and 50 nM NusA416; lane 6, M. tuberculosis nut RNA; lanes 7–9, M. tuberculosis nut RNA with 5, 10, 25, and 50 nM NusA416. All lanes contain 1 nM RNA and 1 M KCl.
