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| Strain | Insert | Read-through (% of max) |
|---|---|---|
| KAM103 | Leader | 86 |
| KAM104 | Leader (U47A) | 78 |
| KAM107 | nut | 100 |
| KAM108 | nut (U47A) | 51 |
| KAM109 | None | 14 |
The 3′/5′ ratio of lacZ RNA determined by slot blotting is shown. The values are expressed as percentages of the maximum value obtained with KAM107 (Prrn—nutwt—lacZ). Raw counts for KAM107 are given in Materials and Methods.
