Figure 2.

Landscape of protective tumor immunity elicited by tumor-specific peptides. (A) Tumor-protective activity of the mutated epitopes with top NetMHC or DAI scores for CMS5 and Meth A. (A and B), Mice were immunized with peptides that had the highest NetMHC scores (A) or top DAI scores (B) and challenged with live tumor cells, and tumor growth monitored. Area under the curve (AUC; Duan et al., 2012) for each individual tumor growth curve was calculated and normalized by setting the naive group to a value of 100, shown by a horizontal red line. Bars corresponding to peptides that show statistically significant tumor-protective immunogenicity are filled in red, and indicated by an asterisk (P = 0.015–0.03). The peptides are arranged in order of increasing antitumor activity. The pie charts show the percentage of neoepitopes tested that did not (black) and did (red) elicit protection from tumor challenge. (C) Examples of tumor growth curves in untreated mice (naive) and mice immunized with indicated mutant peptides from CMS5 or their WT counterparts. Stau1.2 is a representative neoepitope that does not elicit protective immunity, whereas Atxn10.1 and Alkbh6.2 are representative neoepitopes that do. Each line shows the kinetics of tumor growth in a single mouse. The experiments were performed three times.