Table 4.
CMS5 and Meth A epitopes with highest NetMHC PWM scores.
| Gene | Mut/WT sequence | Mut/WT Score | Measured IC50 for Kd (Mut/WT) | ICS | ELISpot | Tumor rejection |
| CMS5 Epitopes with highest NetMHC scores | ||||||
| Ssx2ip | CYAK(v/L)KEQL | 14.5/14.1 | 26/3.2 | - | - | - |
| Mapk1.1 | (q/K)YIHSANVL | 13.2/12.4 | 57/0.2 | - | - | - |
| Farsb | HY(v/L)HIIESKPL | 13/13.6 | 423/52 | + | - | - |
| Ncoa3 | (h/Q)YLQYKQEDL | 11.5/11.7 | 2162/54074 | - | - | - |
| Mapk1.2 | (q/K)YIHSANV | 11.4/10.7 | 2135/295 | - | - | - |
| Mapk1.3 | LYQILRGL(q/K)YI | 11.3/11 | 110/333 | - | +++++ | - |
| Serinc1 | NYLLSLVAV(m/V)L | 11/10.2 | 2679/20861 | - | +++++ | - |
| Meth A Epitopes with the highest NetMHC scores | ||||||
| Usp12 | SY(l/R)VVFPL | 14.2/12.3 | 6835/1155 | - | +++++ | - |
| Tfdp1 | QYSGS(w/R)VETPV | 14.2/15.3 | -a/603 | - | - | - |
| Ufsp2 | HYINM(i/S)LPI | 14.2/14.5 | 0.23/+++b | + | +++++ | - |
| Apc | AYCETCWE(l/W) | 14/8.1 | 23/60 | + | +++++ | - |
| Hspg2 | SY(l/Q)LGSGEARL | 14/14.4 | 2623/79 | - | - | - |
| Ccdc85c | TYIRP(f/L)ETKV | 13.5/13.1 | 6155/118 | - | - | - |
| Pacs2 | HYLS(s/A)ILRL | 13.4/12.7 | 41/1269 | - | - | - |
| Alms1.1 | (l/S)YLDSKSDTTV | 13.3/15.2 | 79/16 | + | - | - |
| Alms1.2 | YYVPLLKRVP(l/S) | 13.3/7.3 | 421/1485 | - | ++ | - |
| Ckap5 | K(y/D)MSMLEERI | 13.2/1.7 | 17/7686 | + | - | - |
| Abr | GYFVSKAKT(s/R)V | 13.1/12.8 | 958/570 | + | - | - |
a
IC50 > 70,000 nM
b
+++, IC50 < 0.1 nM
Note for ELISpot results: 1–9 spots/106 CD8 = +; 10–20 spots/106 CD8 = ++; 21–50 spots/106 CD8 = +++; 51–100 spots/106 CD8 = ++++; >100 spots/106 CD8 = +++++.