Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Oct 20;211(11):2281–2295. doi: 10.1084/jem.20132059

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Marcais et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 7. — Partial correction of Akt S473 phosphorylation, nuclear translocation of NF-κB, and co-stimulation–independent IL-2 production by transient knockdown of Rictor in Dicer-deficient CD4 T cells. (a) siRNA-mediated knockdown of Rictor in Dicer-deficient CD4 T cells affects Rictor but not Raptor expression (mean ± SE; n = 4 independent experiments). (b) Naive CD4Cre Dicer^Δ/Δ and control Dicer^lox/lox CD4 T cells were activated with anti-TCR for 2 h and analyzed by immunoblotting with antibodies to Akt, pAkt Ser473; and Lamin B for normalization (mean ± SE; n = 3 independent experiments; *, P < 0.05). (c) Naive CD4Cre Dicer^Δ/Δ and control Dicer^lox/lox CD4 T cells were activated with anti-TCR for 3 h and the nuclear translocation cRel was determined (ImageStream similarity scores > 0.5; mean ± SE; n = 3 independent experiments). (d) Naive CD4Cre Dicer^Δ/Δ and control Dicer^lox/lox CD4 T cells treated as in a) were activated with anti-TCR or anti-TCR+CD28 and secreted IL-2 was quantified by ELISA after 18 h (mean ± SE; n = 3 independent experiments).