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. 2014 Oct 20;211(11):2281–2295. doi: 10.1084/jem.20132059

Figure 8.

Figure 8.

Functional relevance of IL-2 produced by Dicer-deficient CD4 T cells in the absence of co-stimulation. (a) Resistance of Dicer-deficient CD4 T cells to anti-TCR–induced anergy is contagious. Thy1.1+ control and Thy1.2+ CD4Cre DicerΔ/Δ CD4+ CD25− T cells were cultured either alone or as a mixture (mix) on plate-bound anti-TCR (500 ng/ml, left; graded concentrations, right). IL-2 production was determined 5 h after restimulation with anti-TCR+CD28 using Thy1.1 to distinguish control CD4 T cells from Dicer-deficient CD4 T cells (mean ± SE; n = 2 independent experiments). (b) CD45.1 Il2-deficient CD4 T cells were activated with anti-TCR alone or with anti-CD3+CD28 in the presence of TGF-β (1 ng/ml). Where indicated, cultures were supplemented with CD45.2 control or Dicer-deficient CD4 T cells. Il2-deficient CD4 T cells alone were unable to induce Foxp3. In the presence of co-stimulatory signals, both control and Dicer-deficient CD4 T cells could rescue Foxp3 induction in Il2-deficient CD4 T cells. In the absence of co-stimulation, only Dicer-deficient CD4 T cells were able to facilitate Foxp3 induction in Il2-deficient CD4 T cells (mean ± SEM; n = 3 independent experiments).