Fig. 2.
BMEL-derived hepatocytes and bile ducts localized within a portal triad show appropriate nuclear and cell-type-specific expression of liver-enriched transcription factors. BMEL-GFP cells are recognized as H-2Dd-negative (A), GFP-positive (C and E), or H-2Kk-positive (G). (B and D) HNF1α and HN4α, respectively, revealing nuclear staining of cells within the BMEL cluster of hepatocytes. (F) HNF4α is expressed in hepatocytes but not in bile ducts, and is absent from the BMEL-derived bile duct shown. (H) HNF1β is strongly expressed in bile ducts, including the duct formed by BMEL cells, whereas it is weakly expressed in hepatocytes. In B, D, F, and H, immune cells with small nuclei are visible surrounding the BMEL cell islands. BD, bile duct; A, artery; V, vein. (Scale bars: 100 μm.)