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. 2004 May 17;101(22):8366–8371. doi: 10.1073/pnas.0308758101

Fig. 5.

Fig. 5.

Nucleotide sequence of the region coding for Gsα amino acids Met-221 through Arg-280, which in Homo sapiens are encoded in exons 9 and 10, reveals them to be encoded in a single exon in Mus musculus. (A) The genomic region of interest was amplified by PCR (forward and reverse primers as shown) using genomic DNA from an FVB/N mouse and sequenced by the dideoxynucleotide chain termination method. Shown is the complete sequence of the amplified DNA and the deduced amino acid sequence encoded in the fully spliced RNA. This sequence (only partially shown) spans from the middle of exon 7 to the middle of the intron that follows the exon 9-10 coding region. The sequence shown for the murine gene was identical for DNA from 129SV, FVB/N, BALB/c, and C57BL/6J mice. (B) Human Gnas1 nucleotide and deduced amino acid sequence (ref. 4; GenBank accession no. NT_011362 region). Nucleotide sequences (in blocks of 10): uppercase, coding (exonic); lowercase italic, noncoding (intronic).