Table 2.

Summary of designs of effects tests for eCry3.1Ab

Test organism	Source of eCry3.1Ab	Exposure route	Intended bioassay conditions	Duration (days)	Sample sizea	Guideline or protocol
C. maculata	Microbial	Artificial diet	23–27 °C; 40–80 % RH; 16 h L/8 h D	21	40 × 1	OPPTS 885.4340 (1996c)
O. laevigatus	Microbial	Artificial diet	24.5–26.5 °C; 65–74 % RH; 16 h L/8 h D	12	40 × 1	OPPTS 885.4340 (1996c)
A. bilineata	Microbial	Artificial diet	18–22 °C; 60–90 % RH; 16 h L/8 h D	79b	4 × (10♂ + 10♀)	Grimm et al. (2000)
P. cupreus	Microbial	Artificial diet	19–21 °C; 60–91 % RH; 16 h L/8 h D	57b	40 × 1	Heimbach (1992)
E. fetida	Microbial	Artificial soil	20–22 °C; 49–55 % RH; 24 h L	14	4 × 10	OECD 207 (1984)
A. mellifera	Microbial	Sucrose solution	Field conditions	24b	4 × (100 eggs + 100 larvae)c	Oomen et al. (1992)
				22b	3 × >100 eggsd	OECD 75 (2007), Oomen et al. (1992)
C. virginianus	Microbial	Gelatin capsule	24.1–25.1 °C; 56–72 % RH; 8 h L/16 h D	14b	1 × 5♂, 1 × 5♀	OPPTS 850.2100 (1996b), FIFRA Subdiv E 71-1
M. musculus	Microbial	Gavage	19–25 °C; 30–70 % RH; 12 h L/12 h D	14b	1 × 5♂, 1 × 5♀	OECD 420 (2001)
G. fasciatus	5307 maize leaves	Direct consumption	17–19 °C; 16 h L/8 h D	5	4 × 10	OPPTS 850.1020 (1996a)
I. punctatus	5307 maize grain	Fish feed	23–27 °C; 16 h L/8 h D	28	4 × 20	ASTM (2004)

RH relative humidity; L/D light/dark period each day, OPPTS US EPA Office of Prevention, Pesticides and Toxic Substances [currently known as Office of Chemical Safety and Pollution Prevention (OCSPP)], OECD Organisation for Economic Co-operation and Development, ASTM American Society for Testing and Materials, FIFRA Federal Insecticide, Fungicide and Rodenticide Act (United States)

^aOrganisms per treatment: replicates × individuals per replicate

^bSingle or short-term dose: duration includes observation period after final dosing/exposure

^{c, d}Independent studies