Fig. 2.

Astrocytic candidate gene validation. (A) Candidate astrocyte genes with glial expression based on similarity to the reference gene expression patterns for GFAP ISH and/or GFAP immunohistochemistry were chosen for further validation. Note the marked abundance of GFAP RNA in GL and CC (arrowheads) and relative absence in cortical GM (cx). (B) The majority of validated genes showed a broad “pan-astrocytic” pattern of expression in GM and WM astrocytes [shown here are: Clusterin (Clu), ApoE, GST (GSTm), Aldolase 3 (Aldo3), and Cystatin 3 (Cst3)]; a subset of each which were GFAP-positive. (C) Several validated astrocyte genes showed a restricted expression pattern in subsets of astrocytes. Phospholipase A, group7 (Pla2g7) was predominantly expressed in cortical GM astrocytes, whereas Aquaporin 4 (Aqp4) was highly abundant in GL regions.