Fig. 1.

Pacrg transcript is highly expressed in testis in both mouse and humans. (A) Northern blot on multiple mouse tissues shows that Pacrg mRNA is detected only wild-type testis (testis lane wt). The Pacrg mRNA is completely absent in testes from qk^v mutant animals (testis, qk^v/qk^v lane). (Lower) Hybridization with an actin probe as loading control. (B) Northern blot on human tissues shows that PACRG mRNA is most abundant in testis. Lower levels of expression are also detected in brain, liver, small intestine, pancreas, prostate, and ovaries. (Lower) Hybridization with an actin probe is shown as a loading control.