Fig. 5.
Pacrg is evolutionarily conserved in protozoan species. Protein sequence alignment of mouse Pacrg protein with gene products from protozoa. Residues conserved in the majority of sequences are highlighted in red, conservative amino acid substitutions are highlighted in yellow, and residues identical in all sequences are highlighted in green. The consensus sequence is shown in the lower line of the alignment. The arrowhead indicates the partially conserved cysteine residue within the putative prenylation motif. The protein sequences were obtained by conceptual translation of DNA GenBank and dbEST database entries from each species, corresponding to the following accession nos.: Mouse, AK005771; Chlamydomonas, BG856901; Trichomonas, CD664326; Trypanosoma, AC091330; Giardia, BAB56145; Leishmania, AAF69760.1. Alignment was generated by using clustalw version 1.8 (see Materials and Methods).