Fig. 2.

Expression of MIP-1α in Hexb^+/+ and Hexb^-/- mice. (a) The time course of relative MIP-1α mRNA expression levels in the cerebral cortex and spinal cord of Hexb^-/- mice compared with age-matched Hexb^+/+ mice. (b–g) Immunofluorescent staining of the spinal cord with MIP-1α and GFAP antibodies. (b–d) Control (Hexb^+/+) sections. (e–g) Hexb^-/- sections. b and e show MIP-1α immunostaining. c and f show GFAP immunostaining. d and g show double immunostaining for MIP-1α and GFAP. Note that the MIP-1α and GFAP double-positive astrocytes (arrows in e–g) are closely associated with vessels (*). (Bar: 60 μm.)