Figure 8.
The immunogenicity of NOMV in a murine in vivo model. BALB/c mice (15 per group) were immunized with 5 μg of lgtB, lpxL1/lgtB or lpxL1. Serum was collected at day 42 and analysed for specific IgG antibody by ELISA (A). Wild-type H44/76 and PorA-deficient H44/76 were used as target antigens in the ELISA. Specific IgG levels are expressed as log10 titres for individual mice and the geometric mean is shown for each group. Antibody function was assessed by SBA (B). Briefly, mouse serum was incubated with rabbit complement together with either wild-type H44/76 or PorA-deficient H44/76. Highest dilution that resulted in 90% killing of bacteria is shown for individual mice and the geometric mean for each group. Statistical significance was determined by a paired T-test unless stated otherwise. For SBA against PorA-deficient H44/76 the number of responders are indicated on the graph. A responder was defined as a mouse that who serum killed 90% of bacteria at at least 1:5 dilution. *Statistical significance was determined by Fishers Exact Test.