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. 2014 Aug 11;29(12):1476–1485. doi: 10.1002/mds.25978

FIG 1. Analysis of α-Syn knockdown by various siRNAs in cell models.

FIG 1

SH-SY5Y cells over-expressing mouse α-Syn-HA were treated with 100 nM of three different siRNAs (siRNA 1, 2, or 3) using Hiperfect transfection reagent (A, B) or 3 μg RVG exosomes loaded with the different siRNAs (C, D). After 3 days, total α-Syn mRNA levels were quantified by qPCR relative to GAPDH (A, C) and mouse α-Syn (anti-HA antibody) protein levels relative to actin by Western blot (B, D). Data expressed as mean ± SEM (n = 4). Statistical analyses compared with control group, *p < 0.05, **< 0.01, ***< 0.001.