Fig. 2.

Enhanced neurite outgrowth from exercise-conditioned neurons does not require new mRNA synthesis in vitro. L4–5 DRGs were isolated from sedentary and 7-day-exercised animals and dissociated for culture. The RNA polymerase inhibitor DRB (80 μM) was included in the culture medium to evaluate the requirement for new mRNA synthesis by the exercise conditioned DRGs. After 22 h in vitro, cultures were fixed and stained for neurofilament. Results are displayed as mean neurite length (error bars = 2 × SEM). Student's t test revealed P < 0.0001 for exercise plus DRB cultures vs. sedentary cultures. Comparing exercised neurons cultured with vs. without DRB and sedentary neurons cultured with vs. without DRG showed no statistically significant differences (P > 0.05).