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. 2014 Oct 4;16(3):255–262.

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Fig 3 — Isolation of mesenchymal stem cells (MSCs) from adipose tissue and characterized by differentiation. Panel A shows passage 2 adipose-derived MSCs (AD-MSCs), Panel B represents oil red staining of passage 2 AD-MSCs that differentiated into adipocytes. The vesicle that contained oil is visible in cells which showed adipogenic differentiation and Panel C shows the passage 2 AD-MSCs that were cultured in osteogenic differentiation medium and stained with alizarin red. Alizarin red stained the calcium deposits which confirmed osteogenic differentiation.