Fig 3.

MTT assay for mouse embryonic stem cell (mESCs) proliferation on poly (ε-caprolactone) (PCL) and plasma treated PCL (p-PCL) aligned and random nanofiber scaffolds and gelatin coated tissue culture plate (TCP). Bar represents mean ± standard deviation. *; Significant level of adherance and proliferation on TCP compared to aligned PCL (A-PCL), random PCL (R-PCL), aligned plasma treated PCL (Ap-PCL), random plasma treated PCL (Rp-PCL) on day 1 at ≤0.05, #; Significant level of proliferation on plasma treated random and aligned nanofiber compared to untreated scaffolds and gelatin coated TCP on day 3 at ≤0.05,†; Significant reduction of proliferation level on aligned PCL scaffold compared to the other groups on day 5 at ≤0.05, OD; Optic density, AP; Plasma treated aligned nanofibrous scaffold, RP; Plasma treated random nanofibrous scaffold, A; Nontreated aligned nanofibrous scaffold, R; Nontreated random nanofibrous scaffold and T; Gelatin coated tissue culture plate (TCP).