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. 2014 Oct 4;16(3):299–308.

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Fig 4 — Effect of Silybin and Silybin-phosphatidylcholine on ESR1 expression after 24 (A), 48 (B), and 72 hours (C) of treatment in the T47D breast cancer cell line. The reverse-transcribed RNA and amplified cDNA was normalized for GAPDH expression. Relative expression graphs were depicted by SPSS 18 (simple bar, summaries for group of case). Data were analyzed by the ΔΔCT relative expression method, and presented as two independent experiments. Each experiment had two individual samples (Error bars: ± SD). *; p< 0.05, **; p<0.01 and ***; p< 0.001.