Fig. 1.

10,17S-docosatriene (NPD1) is synthesized in ARPE-19 cells. (A) Elucidation of the structure of NPD1 by lipidomic analysis by LC-MS/MS (33–35). MS/MS spectrum for NPD1 shows a full scan of negative ion products for selected parent ion (m/z 359). (Inset) The UV spectrum. (B) Production of NPD1 by IL-1β (6 h). (C) Calcium ionophore A-23187 (10 μM) promoted the release of free DHA (blue bars) and the formation of NPD1 (red bars) as a function of incubation time. No exogenous DHA was added. As described in Materials and Methods, cells were treated with IL-1β or A-23187, then lipids were extracted and analyzed by LC-MS/MS.